Protein Method Blocking Buffers
LI COR BIOTECH LLC Intercept (PBS) Blocking Buffer (10 x 500mL)
Intercept (PBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. Intercept (PBS) Blocking Buffer is the blocking buffer of choice for near-infrared fluorescent applications when using a phosphate-buffered saline (PBS) system. It contains no mammalian proteins for the lowest cross-reactivity with mammalian antibodies, it is an excellent choice for biotin-streptavidin detection and it is suitable for a wide range of applications, including Western blots, In-Cell Western™ assays, and protein arrays. Intercept (PBS) Blocking Buffer is a ready-to-use blocker formulation in PBS. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection. It is stored at 4 °C and is also available in kits with Immobilon-FL PVDF and nitrocellulose membranes.
Teknova 0.01M PBS with 0.02% Tween-80. 1000mL, Sterile
0.85% Sodium Chloride, 0.12% Sodium Phosphate Dibasic, 0.022% Sodium Phosphate Monobasic, 0.02% Tween-80
Abcam Immunoassay Blocking (BSA Free), 50ML
Abcam’s Immunoassay Blocking (BSA Free) (ab171535) maintains the activity of antibodies and other biomolecules adsorbed or immobilized to solid substrates.
The product is subject to the following: Abcam Restricted Use Statement
IBI SCIENTIFIC 3P 10X PBST (PBS W/0.5% TWEEN 20) 4/PACK
Sterile buffer solution containing sodium phosphate, sodium chloride, potassium phosphate and potassium chloride with 0.5% TWEEN 20.Sterile filtered using 0.1um filterDNase, RNase and protease free.Sterility validated using USP <71> Test for SterilityDoes not contain calcium, magnesium, phenol red or sodium pyruvate. IBI 10X PBST (10X PBS) with TWEEN 20 (0.5% TWEEN 20) is a concentrated sterile solution in a 1 liter bottle that is ideal for use in immunoassay procedures such as Western Blotting and ELISAs in microtiter plate wells.IBI 10X PBST is mixed and bottled in our liquid production facility here in Dubuque, Iowa. Our high output pure water system produces endotoxin free and nuclease free water at a rate of 100 gallons per/hr. This water is the basis for all of our liquid blending production here at IBI.
LI COR BIOTECH LLC Intercept (PBS) Protein-Free Blocking Buffer (3x500mL)
Intercept (PBS) Protein-Free Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. This buffer contains no proteins, for assays where use of animal-based products is prohibited. They are an excellent choice for biotin-streptavidin detection and suitable for a wide range of applications, including Western blots, In-Cell Western™ assays, and protein arrays. Intercept (PBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in phosphate-buffered saline and is stored at 4 °C. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection.
SCANTIBODIES LABORATORY INC Heterophilic Blocking Tube
This item must be ordered in multiples of 50 per supplier requirements
Tube, Blocking; Heterophilic; Unique blocking reagent composed of specific binders which inactivate heterophilic antibodies; Allows rapid and simple elimination of false positive heterophilic interference in plasma for sandwich immunoassays.
Cell Biolabs Inc PhosphoBLOCKER™ Blocking Reagent, 200 g dry blend (4 Liters prepared)
The utilization of anti-phosphoprotein antibodies in western blotting has become a commonly used tool for signal transduction research. Unfortunately, low levels of endogenous phosphoprotein in various cell lysates often can not be detected, even with high concentrations of antibody and long exposure times. Most commercially available western blot blockers (e.g. dry milk, serum) are sufficient to block the unreacted sites on the membrane, reducing the amount of nonspecific antibody binding during the assay; however, they are not designed to preserve phosphoprotein antigens during blotting. Cell Biolabs’ PhosphoBLOCKER™ contains a proprietary formulation that provides several advantages over conventional blockers: (1) It is designed specifically for phosphoprotein blotting; (2) It enhances low level phosphoprotein signal without increasing background; and (3) it is an easy to use premixed dry blend. 200 grams (reconstituted to 4 liters).
BBS, MP Biomedicals™
Serves as a general purpose loading dye. MP Biomedicals BBS can be used with neutral DNA or RNA agarose gels.
MilliporeSigma™ Immunoblot Blocking Reagent
For use in western blots as a blocking agent
MilliporeSigma™ Novagen™ 5% Alkali - Soluble Casein
Used as blocking reagent for Western blot applications.
Teknova 20X TBS with 1% Tween-20, pH 7.5. (2M Tris, 3M NaCl) 1000mL, Sterile. 8 pack.
20X TBS with 1% Tween-20, pH 7.5. (2M Tris, 3M NaCl) 1000mL, Sterile. 8 pack.